Prolonged duration of persistent cell-free fetal DNA from vanishing twin
نویسندگان
چکیده
منابع مشابه
Introducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA
Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
متن کاملevaluation of a modified dna extraction method for isolation of cell-free fetal dna from maternal serum
background: discovery of short cell free fetal dna (cffdna) fragments in maternal plasma has created major changes in the field of prenatal diagnosis. the use of cffdna to set up noninvasive prenatal test is limited due to the low concentration of fetal dna in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. the aim of this study was to ev...
متن کاملFetal fraction estimate in twin pregnancies using directed cell-free DNA analysis.
OBJECTIVE To estimate fetal fraction (FF) in monozygotic and dizygotic twin pregnancies. METHODS Maternal plasma samples were obtained from 35 monochorionic twin pregnancies with male fetuses (monozygotic) and 35 dichorionic pregnancies discordant for fetal sex (dizygotic) at 11-13 weeks' gestation. Cell-free DNA was extracted and chromosome-selective sequencing with digital analysis of selec...
متن کاملCell-free fetal DNA in celomic fluid.
1. Minniti S, Vincentini S, Procacci C. Congenital anomalies of the venae cavae: embryological origin, imaging features and report of three new variants. Eur Radiol 2002; 12: 2040–2055. 2. Nsah EN, Moore GW, Hutchins GM. Pathogenesis of persistent left superior vena cava with a coronary sinus connection. Pediatr Pathol 1991; 11: 261–269. 3. Pasquini L, Belmar C, Seale A, Gardiner HM. Prenatal d...
متن کاملCell‐free fetal DNA: the new tool in fetal medicine
Since the discovery of cell-free fetal DNA (cffDNA) in maternal plasma in 19971 there has been rapid progress in harnessing this as a source of fetal genetic material for prenatal diagnosis. The majority of cell-free DNA (cfDNA) is maternal in origin2, with the fetal proportion emanating from the placenta3, detectable in the maternal circulation from around 5 weeks’ gestation2 and constituting ...
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ژورنال
عنوان ژورنال: Ultrasound in Obstetrics & Gynecology
سال: 2018
ISSN: 0960-7692
DOI: 10.1002/uog.19004